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Pinus krempfii Lecomte is morphologically very unique as
compared to other Pinus species by having flat leaf-like needles.
Its taxonomic position has been problematic ever since its discovery.
In this study, an attempt was made to infer the taxonomic status of
P. krempfii through restriction fragment length polymorphism
(RFLP) analysis of 12 PCR amplified chloroplast (cp) DNA regions.
Phylogenetic analysis was conducted using 10 representatives of the
two Pinus subgenera: Strobus and Pinus.
In addition, to infer the position of P. krempfii in Pinaceae
in relation with other genera, 14 representatives of eight additional
genera were included in the analysis. Our cpDNA-based results indicate
that: 1) P. krempfii clearly belongs to the genus Pinus.
This result does not favour the creation of a new genus
Ducampopinus in Pinaceae for this taxon;
2) Within the genus Pinus, P. krempfii is more allied
with species in subgenus Strobus and differs distinctly from
species in subgenus Pinus; 3) Despite the similarity in
certain morphological and anatomical leaf and wood characters to
Keteleeria and Pseudolarix, the cpDNA data do not
support the hypothesis for close relationship between P. krempfii
and these two genera.
Key words: Gymnosperms, Pinaceae, Pinus, cpDNA, PCR-RFLP, Pinus krempfii, phylogeny.
by Alfred E. Szmidt