For reprint send email to: Alfred E. Szmidt
We used PCR-RFLP analysis of the chloroplast DNA of
the genus Abies (family Pinaceae), to determine
if the method could be used to detect inter-specific
variation in this genus and to study how the variation
was distributed in different regions of this genome.
Ten different cpDNA regions, consisting of coding and
non-coding DNA sequences, were amplified with specific
primers in ten different Abies taxa. The
amplification products were digested with several
restriction enzymes. Results showed that the
chloroplast genome is highly variable in most of the
investigated taxa and contains multiple variable
regions that appear to be distributed throughout the
whole genome. Species-diagnostic markers were found
for four of the ten investigated species. Unexpectedly
intra-specific variation was also detected in four species.
It is likely that further studies including larger
sample sizes and/or more powerful methods for the
detection of chloroplast DNA variation will reveal
additional cpDNA variation for this genus.
Key words: Abies, chloroplast DNA, evolution, PCR-RFLP
by Alfred E. Szmidt