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The Laboratory for Animal Developmental Biology and Reproductive Engineering

Department of Biology, Faculty of Science, Kyushu University




New information:

2019.1.1 Daisuke Saito took up the professor of laboratory for Animal Developmental Biology and Reproductive Engineering on the 1st January 2019.



Research:

Germ cells are the sole and fundamental cells to leave offspring. Although germ cells in an animal body would be strictly regulated to avoid aberrant proliferation, cell death, and differentiation due to its biological significance, the regulatory mechanisms of germ cells have been largely unknown. To address the issues, we study the developmental mechanisms of germ cells in vertebrate embryos, focusing on the cellular and molecular interactions between germ cells and environmental somatic cells, in the contexts of germ cell maintenance and migration. We progress these researches by maximizing the advantages of avian system including in vivohigh quality imaging, cell/tissue transplantation, germ cell culture, and transgenic chickens and quails. In addition to basic developmental researches, we are also interested in various applications of avian reproductive engineering.





Publications:

Saito, D., Tamura, K. and Takahashi, Y.: Early segregation of the adrenal cortex and gonadin chicken embryos. Dev. Growth Differ. 59: 593-602, 2017
 
Matsubara, H., Saito, D., Abe, G., Yokoyama, H., Suzuki T., and Tamura, K.: Upstream regulation for initiation of restricted Shh expression in the chick limb bud. Developmental Dynamics 246: 417-430, 2017
 
Seki, R., Li, C., Fang, Q., Hayashi, S., Egawa, S., Hu, J., Xu, L., Pan, H., Kondo, M., Sato, T., Matsubara, H., Kamiyama, N., Kitajima, K., Saito, D., Liu, Y., Gilbert, M.T.P., Zhou, Q., Xu, X., Shiroishi, T., Irie, N., Tamura, K., and Zhang, G.: Functional roles of Aves class-specific cis-regulatory elements on macroevolution of bird-specific features. Nat. Commun. 8: 14229, 2017
 
Yoshino, T., Murai, H., and Saito, D.: Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos. Nat. Commun. 7: 12561, 2016
 
Seki, R., Kitajima, K., Matsubara, H., Suzuki, T., Saito, D., Yokoyama, H. and Tamura, K.: AP-2β is a transcriptional regulator for determination of digit length in tetrapods. Dev. Biol. 407: 75-89, 2015
 
Saito, D. and Takahashi, Y.: Sympatho-adrenal morphogenesis regulated by the dorsal aorta. Mechanism of Development S0925-4773 (15) 30013-30017, 2015 (Review)
 
Takahashi, T., Takase, Y., Yoshino, T., Saito, D., Tadokoro, R. and Takahashi, Y.: Angiogenesis in the developing spinal cord: Blood vessel exclusion from neural progenitor region is mediated by VEGF and its Antagonists. PLoS One 10: e0116119, 2015
 
Yoshino, T., Saito, D., Atsuta, Y., Uchiyama, C., Ueda, S., Sekiguchi, K. and Takahashi, Y.: Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet. Proc. Natl. Acad. Sci. USA 111: 6660-6665, 2014
 
Atsuta, Y., Tadokoro, R., Saito, D.and Takahashi, Y.: Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo. Dev. Growth Differ. 55: 579-590, 2013
 
Saito, D., Takase, Y., Murai, H. and Takahashi, Y.: The dorsal aorta initiates a molecular cascade that instructs sympatho-adrenal specification. Science 336: 1578-1581, 2012
 
Yokota, Y., Saito, D., Tadokoro, R. and Takahashi, Y.: Genomically integrated transgenes are stably and conditionally expressed in neural crest cell-specific lineages. Dev. Biol. 353: 382-395, 2011
 
Yoshino, T., Saito, D., Tadokoro, R. and Takahashi, Y.: In vivo gene manipulations of epithelial cell sheets: anovel model to study epithelial-to-mesenchymal transition. Dev. Growth Differ. 53: 378-388, 2011
 
Ohata, E., Tadokoro, R., Sato, Y., Saito, D.and Takahashi, Y.: Notch signal is sufficient to direct an endothelial conversion from non-endothelial somitic cells conveyed to the aortic region by CXCR4. Dev. Biol. 335: 33-42, 2009
 
Watanabe, T., Sato, Y., Saito, D., Tadokoro, R. and Takahashi, Y.: EphrinB2 cordinates the formation of a morphological boundary and cell epithelialization during somite segmentation. Proc. Natl. Acad. Sci. USA 106: 7467-7472, 2009
 
Sato, Y., Watanabe, T., Saito, D., Takahashi, T., Yoshida, S., Kohyama, J., Ohata,, E., Okano, H. and Takahashi, Y.: Notch signaling mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos. Dev. Cell 14: 890-901, 2008
 
Watanabe, T., Saito, D., Tanabe, K., Suetsugu, R., Nakaya, Y., Nakagawa, S. and Takahashi, Y.: Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos. Dev. Biol. 305: 625-636, 2007
 
Saito, D., Yonei-Tamura, S., Takahashi, Y. and Tamura, K.: Level-specific role of paraxial mesoderm in regulation of Tbx5/Tbx4 expression and limb initiation. Dev. Biol. 292: 79-89, 2006
 
Tamura, K., Amano, T., Sato, T., Saito, D., Yonei-Tamura, S. and Yajima, H.: Expression of rigf, a member of avian VEGF family, correlates with vascular patterning in the developing chick limb bud. Mechanism of Development 120: 199-209, 2003
 
Saito, D., Yonei-Tamura, S., Kano, K., Ide, H. and Tamura, K.: Specification and determination of limb identity: evidence for inhibitory regulation of Tbx gene expression. Development 129: 211-220, 2002
 
Tamura, K., Kuraishi, R., Saito, D., Masaki, H., Ide, H., and Yonei-Tamura, S.: Evolutionary aspect of positioning and identification of vertebrate limbs. Journal of Anatomy 199: 195-204, 2001 (Review)



Members:



Professor Daisuke Saito Ph.D




Contact:

Grad. Sch. Sys. Life Sci.
Kyushu University
744 Motooka
Nishi-ku 819-0395
Japan
Tel and FAX: +81-092-802-4270
e-mail: saito.daisuke.036(at-mark)m.kyushu-u.ac.jp

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